Membrane 126 kilodalton phospho glyco protein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells

Filed under: Epithelium

Edgington T.S., 1983: Membrane 126 kilodalton phospho glyco protein associated with human carcinomas identified by a hybridoma antibody to mammary carcinoma cells. Proceedings Of The National Academy Of Sciences Of The United States Of America: 1332-1336

A large number of hybridomas producing antibodies to the membrane fraction of a human mammary carcinoma cell line were produced. Among those hybridoma antibodies that bound to exposed surface membrane epitopes on the human mammary carcinoma cell line Bt-20, one bound to all tested breast carcinoma cell lines and to a number of other human carcinomas by solid-phase radioimmunoassay. This hybridoma antibody, designated 10-3d2, does not bind to normal mammary epithelium, a variety of control cell lines, or selected normal human tissue homogenates. The 10-3d2 hybridoma antibody identifies by immunoprecipitation a membrane 126-kilodalton protein which is indistinguishable in different tumor cells. It is glycosylated by reference to incorporation of glucosamine and also is phosphorylated by reference to incorporation of 32p. It is present in a finely granular pattern on the cell surface of mammary carcinoma cells with concentration at the sites of apparent cell-cell contact. This 126-kilodalton phosphoglycoprotein, which could not be identified by immunoprecipitation of intrinsically labeled normal cells, may represent a heretofore unrecognized tumor-associated marker for human mammary carcinomas and certain other types of human neoplasms.




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