Also known as the ELISA, the process of enzyme-linked immunosorbent assay is a popular format of analytic biochemisty assay that uses a single subtype of heterogeneous solid-phase enzyme immunoassay (also known as EIA) to detect the presence of an antigen (or a specific substance) in a wet or liquid sample.
The enzyme-linked immunosorbent assay is typically used as a diagnostic tool in medicine and plant pathology, and a quality-control checking method in various industries that involve biochemistry. The process of enzyme-linked immunosorbent assay entails the attempt to both detect and quantify the presence of an antigen in a sample by following a specific set of steps. First, the antigens from the sample are attached to a surface. A further specific antibody will then be applied over the surface to help it bind to the antigen. The antibody used in the process is linked to an enzyme, and in the final step of the process, to a substance with the enzyme’s substrate. The process will result to a subsequent reaction, which produces a detectable signal—typically a color change in the enzyme’s substrate that was added in the final step.
There are different types of enzyme-linked immunosorbent assay: the indirect, the sandwich, multiple and portable, and the competitive ELISA. These types of assay processes can be used for various purposes, and are typically available in kits that are relatively easy to use.
The ELISA can also be used to detect and evaluate the process of an antibody in the sample, which means that it can be used to determine serum antibody concentrations in tests for the HIV or the West Nile Virus. Food industries also rely in this test to detect potential allergens in items such as milk, peanuts, almonds, eggs, and walnuts.
This category contains scientific information of the enzyme-linked immunosorbent assay, a type of test that determines the antigen and antibody concentration in a certain sample.
Estevez, J.; Leiro, J.; Toranzo, A. E.; Barja, J. L.; Ubeira, F. M., 1994: Kinetics of antibody production against Vibrio anguillarum antigens in turbot. Aquaculture. 123(3-4): 196 We evaluated the kinetics of antibody production by turbot (Scophthalmus maximus L.) in response to intraperitoneal (ip) inoculation with Vibrio anguillarum (Atcc strain 19264) cells in the presence [...]
Benfeldt, Connie; Larsen, Lotte B.; Rasmussen, Jan T.; Andreasen, Peter A.; Petersen, Torben E., 1995: Isolation and characterization of plasminogen and plasmin from bovine milk. International Dairy Journal. 5(6): 577-592 A procedure was developed for the isolation of plasminogen and plasmin from bovine milk. Pure undegraded plasminogen with N-terminal amino acid sequence and mobility in [...]
Vigario, A. M.; Eggelte, T. A.; Costa, A.; Seixas, E.; Rosario, V. E., 1994: Investigation of chloroquine intake in Biombo region, Guinea Bissau. Applied Parasitology. 35(4): 237-241 The use of chloroquine (Cq), during the dry season, was determined for 236 patients from 5 villages in Biombo, Republic of Guinea Bissau, West Africa. The antimalarial drug [...]
Sharma, H.; Ohm, H.; Goulart, L.; Lister, R.; Appels, R.; Benlhabib, O., 1995: Introgression and characterization of barley yellow dwarf virus resistance from Thinopyrum intermedium into wheat. Genome. 38(2): 406-413 Wheatgrasses (species of Agropyron complex) have previously been reported to be resistant to barley yellow dwarf virus (Bydv). To introgress this resistance into wheat, Triticum [...]
Buettner, M.; Czerny, C.P.; Lehner, K.H.; Wertz, K., 1995: Interferon induction in peripheral blood mononuclear leukocytes of man and farm animals by poxvirus vector candidates and some poxvirus constructs. Veterinary Immunology & Immunopathology. 46(3-4): 237-250 Prototypes of three poxvirus genera – orthopoxvirus (Opv), parapoxvirus (Ppv), avipoxvirus (Apv) – and Newcastle disease virus (Ndv) as a [...]
Hirabayashi, Tatsuji; Ochiai, Hiroshi; Sakai, Shinya; Nakajima, Katsuhisa; Terasawa, Katsutoshi, 1995: Inhibitory effect of ferulic acid and isoferulic acid on murine interleukin-8 production in response to influenza virus infections in vitro and in vivo. Planta Medica. 61(3): 221-226 We investigated the effect of ferulic acid (Fa) and isoferulic acid (Ifa), which are active components of [...]
Bhattacharya, Dipanwita; Dhar, T. K.; Siddiqui, K. A. I.; Ali, E., 1994: Inhibition of seed germination by Macrophomina phaseolina is related to phaseolinone production. Journal Of Applied Bacteriology. 77(2): 129-133 The production of phaseolinone, a phytotoxic metabolite of Macrophomina phaseolina in infected Phaseolus mungo seeds grown on soil, was estimated by enzyme-linked immunosorbent assay and [...]
Wai, T; Grumet, R., 1995: Inheritance of resistance to the watermelon strain of papaya ringspot virus in the cucumber line TMG-1. HortScience: a ication of the American Society for Horticultural Science 30(2): 338-340 The inbred cucumber (Cucumis sativus L.) line Tmg-1 is resistant to three potyviruses: zucchini yellow mosaic virus (Zymv), watermelon mosaic virus (Wmv), [...]
Yoshida, Toshio, 1993: Inheritance of immunity to citrus tristeza virus of trifoliate orange in some citrus intergeneric hybrids. Bulletin Of The Fruit Tree Research Station. 0(25): 33-43 It was demonstrated that the immunity of trifoliate orange (Poncirus trifoliata (L.) Raf.) to citrus tristeza (Ctv) is controlled by a single dominant gene. Therefore, I investigated whether [...]
Pritchard, Todd J.; Beliveau, Constance M.; Flanders, Kathy J.; Donnelly, Catherine W., 1994: Increased incidence of Listeria species in dairy processing plants having adjacent farm facilities. Journal Of Food Protection. 57(9): 770-775 The processing environments of 30 dairy facilities were surveyed for the presence of Listeria species. Two different primary enrichment media – University of [...]