Also known as the ELISA, the process of enzyme-linked immunosorbent assay is a popular format of analytic biochemisty assay that uses a single subtype of heterogeneous solid-phase enzyme immunoassay (also known as EIA) to detect the presence of an antigen (or a specific substance) in a wet or liquid sample.
The enzyme-linked immunosorbent assay is typically used as a diagnostic tool in medicine and plant pathology, and a quality-control checking method in various industries that involve biochemistry. The process of enzyme-linked immunosorbent assay entails the attempt to both detect and quantify the presence of an antigen in a sample by following a specific set of steps. First, the antigens from the sample are attached to a surface. A further specific antibody will then be applied over the surface to help it bind to the antigen. The antibody used in the process is linked to an enzyme, and in the final step of the process, to a substance with the enzyme’s substrate. The process will result to a subsequent reaction, which produces a detectable signal—typically a color change in the enzyme’s substrate that was added in the final step.
There are different types of enzyme-linked immunosorbent assay: the indirect, the sandwich, multiple and portable, and the competitive ELISA. These types of assay processes can be used for various purposes, and are typically available in kits that are relatively easy to use.
The ELISA can also be used to detect and evaluate the process of an antibody in the sample, which means that it can be used to determine serum antibody concentrations in tests for the HIV or the West Nile Virus. Food industries also rely in this test to detect potential allergens in items such as milk, peanuts, almonds, eggs, and walnuts.
This category contains scientific information of the enzyme-linked immunosorbent assay, a type of test that determines the antigen and antibody concentration in a certain sample.
Boutin B.K., 1983: Food borne entero toxigenic escherichia coli detection and enumeration by dna colony hybridization. Applied & Environmental Microbiology: 1324-1330 Four methods were compared for detecting heat-labile toxin production by E. coli: Dna colony hybridization, 2 enzyme-linked immunosorbent assays and the mouse Y-1 adrenal cell reaction. Although results of the methods were in general [...]
Yoshida H., 1984: Food allergy in atopic dermatitis measurement of allergen specific immunoglobulin e and immunoglobulin g 4 antibodies by using radioallergosorbent test and enzyme linked immunosorbent assay elisa. Hifu: 611-616 Allergen-specific IgE and IgG4 antibodies against 13 food allergen extracts (wheat, rice, corn, buckwheat, peanut, egg white, cow’s milk, pork, codfish, tunny, salmon, crab [...]
The T.H., 1986: Fluctuations in anti nuclear ribonucleoprotein levels in patients with mixed connective tissue disease are related to disease activity as part of a polyclonal b cell response. Annals Of The Rheumatic Diseases: 800-808 In a follow-up study of 11 patients with mixed connective tissue disease the levels of antibodies to nuclear ribonucleoprotein (nRNP) [...]
Suzuki T., 1985: Fimbriae from the oral anaerobe bacteroides gingivalis physical chemical and immunological properties. Journal Of Bacteriology: 730-734 Circular dichroism spectra indicated the predominance of.beta.-sheet structure in B. gingivalis fimbriae regardless of the presence of sodium dodecyl sulfate. By using a computer program, the.alpha.-helix,.beta.-sheet and.beta.-turn contents and the remainder were estimated to be 0, [...]
Rochow W.F., 1979: Field variants of barley yellow dwarf virus detection and fluctuation during 20 years. Phytopathology: 655-660 All 181 isolates of barley yellow dwarf virus (Bydv) that were recovered from field-collected oats, wheat or barley during 1975 and 1976 resembled one of 5 characterized isolates found previously. Most of them resembled Pav, an isolate [...]
Cespedes E., 1986: Field evaluation of elisa for the serodiagnosis of tuberculosis. American Review Of Respiratory Disease: 662-665 An enzyme-linked immunosorbent assay (Elisa) was evaluated as a serodiagnostic test for active tuberculosis in La Paz, Bolivia. Elisa was compared with sputum smear in 277 persons presenting to the Instituto de Torax and was used for [...]
Stewart W.C., 1981: Field evaluation of enzyme linked immuno sorbent assay for detection of antibody to african swine fever virus. American Journal Of Veterinary Research: 1441-1443 The enzyme-linked immunosorbent assay (Elisa) for detection of antibodies to African swine fever virus was evaluated under field conditions in the Dominican Republic. A total of 3402 swine sera [...]
Bharti M.S., 1984: Field evaluation of elisa enzyme linked immunosorbent assay using wuchereria bancrofti mf es antigen for bancroftian filariasis. Bulletin Of The World Health Organization: 941-944 An enzyme-linked immunosorbent assay using W. bancrofti microfilarial excretory-secretory antigen was used in field studies to screen blood samples collected on filter-paper from persons residing in areas endemic [...]
“Hockmeyer W.T., 1987: Field evaluation of an elisa for plasmodium falciparum sporozoite detection in anopheline mosquitoes from kenya. American Journal Of Tropical Medicine & Hygiene: 459-468 An enzyme-linked immunosorbent assay (Elisa) using a monoclonal antibody that recognizes a repetitive epitope on the circumsporozoite protein of Plasmodium falciparum was used in Kenya to assess malaria infections [...]
Collen D., 1987: Fibrinolytic response and fibrin fragment d dimer levels in patients with deep vein thrombosis. Thrombosis & Haemostasis: 1025-1029 An enzyme-linked immunosorbent assay for fragment D-dimer was developed with the use of two monoclonal antibodies directed against specific non-overlapping antigenic determinants, present in fragment D-dimer of crosslinked fibrin but not in fragment D [...]