Also known as the ELISA, the process of enzyme-linked immunosorbent assay is a popular format of analytic biochemisty assay that uses a single subtype of heterogeneous solid-phase enzyme immunoassay (also known as EIA) to detect the presence of an antigen (or a specific substance) in a wet or liquid sample.
The enzyme-linked immunosorbent assay is typically used as a diagnostic tool in medicine and plant pathology, and a quality-control checking method in various industries that involve biochemistry. The process of enzyme-linked immunosorbent assay entails the attempt to both detect and quantify the presence of an antigen in a sample by following a specific set of steps. First, the antigens from the sample are attached to a surface. A further specific antibody will then be applied over the surface to help it bind to the antigen. The antibody used in the process is linked to an enzyme, and in the final step of the process, to a substance with the enzyme’s substrate. The process will result to a subsequent reaction, which produces a detectable signal—typically a color change in the enzyme’s substrate that was added in the final step.
There are different types of enzyme-linked immunosorbent assay: the indirect, the sandwich, multiple and portable, and the competitive ELISA. These types of assay processes can be used for various purposes, and are typically available in kits that are relatively easy to use.
The ELISA can also be used to detect and evaluate the process of an antibody in the sample, which means that it can be used to determine serum antibody concentrations in tests for the HIV or the West Nile Virus. Food industries also rely in this test to detect potential allergens in items such as milk, peanuts, almonds, eggs, and walnuts.
This category contains scientific information of the enzyme-linked immunosorbent assay, a type of test that determines the antigen and antibody concentration in a certain sample.
Vray B., 1987: Pregnancy and humoral immune response in mice chronically infected by trypanosoma cruzi. Infection & Immunity: 2496-2501 The effect of pregnancy on the humoral immune response induced by Trypanosoma cruzi was studied in groups of chronically infected and pregnant mice (Ip) or chronically infected and nonpregnant mice (Inp) of strain Balb/c. Groups of [...]
Greenwood J.M., 1988: Predominance of the ac variant in k88 positive escherichia coli isolates from swine. Journal Of Clinical Microbiology: 149-150 Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all [...]
Chiverton, P. A., 1987: Predation of rhopalosiphum padi homoptera aphididae by polyphagous predatory arthropods during the aphids pre peak period in spring barley. Annals of Applied Biology 111(2): 257-270 Polyphagous predators (:e.g. Aranaea, Carabidae and Staphylinidae), collected from spring barley fields during 1981-85, were examined by either gut dissection or a R. padi-specific antiserum in [...]
Cochrane A.H., 1986: Potential vectors of malaria and their different susceptibility to plasmodium falciparum and plasmodium vivax in northern brazil identified by immunoassay. American Journal Of Tropical Medicine & Hygiene: 873-881 During the period from May 1983 to July 1985 were conducted an epidemiological study to determine potential vectors of malaria in 6 districts in [...]
Pohl L.R., 1988: Potential metabolic basis for enflurane hepatitis and the apparent cross sensitization between enflurane and halothane. Drug Metabolism & Disposition: 135-140 Clinical case reports of unexplained hepatic dysfunction following enflurane and isoflurane anesthesia led to the hypothesis that oxidative metabolism of these drugs by cytochromes P-450 produces immunoreactive, covalently bound acylated protein adducts [...]
Gugerli P., 1980: Potato leaf roll virus concentration in the vascular region of potato tubers examined by enzyme linked immuno sorbent assay. Potato Research: 137-141 Enzyme-linked immunosorbent assay (Elisa), used in conjunction with a new rapid extraction method, showed that potato leafroll virus (Plvr) concentration in the vascular region of infected potato tubers decreases from [...]
Blake E.T., 1985: Postcoital detection of a male specific semen protein application to the investigation of rape. New England Journal Of Medicine: 338-343 Identification of semen in vaginal fluid may provide documentation of sexual contact in alleged victims of rape. An enzyme-linked immunosorbent assay for a semen glycoprotein of prostatic origin, designated p3, is described. [...]
Thayer, W. R.; Coutu, J. A.; Chiodini, R. J.; Van-Kruiningen, H. J.; Merkal, R. S., 1984: Possible role of mycobacteria in inflammatory bowel disease mycobacterial antibodies in crohns disease. Digestive Diseases and Sciences 29(12): 1080-1085 An unclassified Mycobacterium species was isolated from 2 patients with Crohn’s disease (Cd). Antibodies to the unclassified mycobacteria cross-reacted with [...]
Meyer U., 1985: Possible applications and actual use of elisa enzyme linked immunosorbent assay in virus diagnosis in veterinary medicine. Monatshefte Fuer Veterinaermedizin: 186-189 An account is given of possible applications of a sandwich technique and an indirect technique of Elisa in veterinary virology. The development of the method is explained by specific examples.
Margreth A., 1982: Polymorphism of myosin light chains an electrophoretic and immunological study of rabbit skeletal muscle myosins. Biochemical Journal: 529-540 Antibodies specific for rabbit fast-twitch muscle myosin Lc1f L chain were purified by affinity chromatography and characterized by both noncompetitive and competitive enzyme-linked immunosorbent assay (Elisa) and a gel electrophoresis-derived assay (Gedelisa). The antibodies [...]