Also known as the ELISA, the process of enzyme-linked immunosorbent assay is a popular format of analytic biochemisty assay that uses a single subtype of heterogeneous solid-phase enzyme immunoassay (also known as EIA) to detect the presence of an antigen (or a specific substance) in a wet or liquid sample.
The enzyme-linked immunosorbent assay is typically used as a diagnostic tool in medicine and plant pathology, and a quality-control checking method in various industries that involve biochemistry. The process of enzyme-linked immunosorbent assay entails the attempt to both detect and quantify the presence of an antigen in a sample by following a specific set of steps. First, the antigens from the sample are attached to a surface. A further specific antibody will then be applied over the surface to help it bind to the antigen. The antibody used in the process is linked to an enzyme, and in the final step of the process, to a substance with the enzyme’s substrate. The process will result to a subsequent reaction, which produces a detectable signal—typically a color change in the enzyme’s substrate that was added in the final step.
There are different types of enzyme-linked immunosorbent assay: the indirect, the sandwich, multiple and portable, and the competitive ELISA. These types of assay processes can be used for various purposes, and are typically available in kits that are relatively easy to use.
The ELISA can also be used to detect and evaluate the process of an antibody in the sample, which means that it can be used to determine serum antibody concentrations in tests for the HIV or the West Nile Virus. Food industries also rely in this test to detect potential allergens in items such as milk, peanuts, almonds, eggs, and walnuts.
This category contains scientific information of the enzyme-linked immunosorbent assay, a type of test that determines the antigen and antibody concentration in a certain sample.
“Weits J., 1988: Pneumococcal antibodies measured with a rapid enzyme linked immunosorbent assay elisa using the whole vaccine as antigen suggestions for clinical application. Netherlands Journal Of Medicine2: 52-59 This article describes an easy method for estimating the response to vaccination with Pneumovax containing 14 types of pneumococcal capsular polysaccharide. For this purpose, 10 healthy [...]
Saucerman S., 1988: Platelet igg iga igm and albumin correlation of platelet and plasma concentrations in normal subjects and in patients with itp or dysproteinemia. Blood: 362-365 IgG, IgA, IgM, and albumin are primarily known as plasma proteins. Their presence in platelets is poorly understood. The total platelet content of IgG, IgA, and albumin, measured [...]
Pretorius F.J., 1983: Platelet bound immuno globulin m in auto immune thrombocytopenia. Blood: 119-124 Elevated levels of platelet-bound IgG (Pa-IgG) are a feature of autoimmune thrombocytopenia (Atp), but this does not occur in all cases. The role of platelet-bound IgM (Pa-IgM) in these patients was investigated by using a quantitative enzyme-linked immunosorbent assay (Elisa). Determinations [...]
Colman R.W., 1985: Platelet activated complement c 1 inhibitor a secreted alpha granule protein. Journal Of Clinical Investigation: 242-250 In order to characterize which proteins of the contact phase of coagulation intreact with platelets, human platelets were studied immunochemically and functionally to determine if they contain C.hivin.1 inhibitor. By means of monospecific antibody to C.hivin.1 [...]
Hockmeyer W.T., 1987: Plasmodium falciparum immunogenicity of circumsporozoite protein constructs produced in escherichia coli. Experimental Parasitology: 166-172 The immunogenicity of Plasmodium falciparum recombinant circumsporozoite protein constructs R16tet32, and R48tet32 in mice was examined by measuring antibody responses by enzyme linked immunosorbent assay, immunofluorescence, circumsporozoite precipitation, and inhibition of sporozoite invasion. All three constructs were found [...]
Dano K., 1987: Plasminogen activator inhibitor type 1 biosynthesis and messenger rna level are increased by dexamethasone in human fibrosarcoma cells. Molecular & Cellular Biology: 3021-3025 Dexamethasone increases type 1 plasminogen activator inhibitor (Pai-1) activity released from the human fibrosarcoma cell line Ht-1080. We demonstrated that dexamethasone caused about 10-fold increases in the intracellular and [...]
Shibata A., 1988: Plasmin alpha 2 plasmin inhibitor complex in plasma of patients with disseminated intravascular coagulation. American Journal Of Hematology: 162-166 Plasmin-.alpha.2-plasmin inhibitor (.alpha.2pi) complex, an indicator of in vivo plasmin generation, was measured in the plasma of 48 patients with disseminated intravascular coagulation (Dic), by enzyme-linked immunosorbent assay (Elisa). Plasmin-.alpha.2pi complex was markedly [...]
Handin R.I., 1985: Plasmin effect on platelet glycoprotein ib von willebrand factor interactions. Blood: 32-40 The effect of streptokinase on platelets in platelet-rich plasma (Prp) and of plasmin on washed platelets was studied. By 3 1/2 min after the addition of 50,000 Iu/ml streptokinase to Prp, the maximum rate of ristocetin-induced platelet agglutination declined 40%, [...]
Fiorini G.F., 1987: Plasma fibronectin and microvascular damage in essential mixed cryoglobulinemia. Rheumatology International: 213-216 Plasma fibronectin (Fn) was measured in 17 patients with essential mixed cryoglobulinaemia (Emc) and in 17 normal subjects by single radial immunodiffusion (Rid) and enzyme-linked immunosorbent assay (Elisa). In 9 patients the presence of Fn in the cryoprecipitates was also [...]
Cooper J.I., 1985: Plant virus detection using a new form of indirect elisa. Journal Of Virological Methods: 309-320 A novel form of indirect enzyme-linked immunosorbent assay (Elisa) has been devised for the detection of viruses in plants. The method use protein A in two applications to sandwich antibody-antigen-antibody layers. The first applied layer of protein [...]