Also known as the ELISA, the process of enzyme-linked immunosorbent assay is a popular format of analytic biochemisty assay that uses a single subtype of heterogeneous solid-phase enzyme immunoassay (also known as EIA) to detect the presence of an antigen (or a specific substance) in a wet or liquid sample.
The enzyme-linked immunosorbent assay is typically used as a diagnostic tool in medicine and plant pathology, and a quality-control checking method in various industries that involve biochemistry. The process of enzyme-linked immunosorbent assay entails the attempt to both detect and quantify the presence of an antigen in a sample by following a specific set of steps. First, the antigens from the sample are attached to a surface. A further specific antibody will then be applied over the surface to help it bind to the antigen. The antibody used in the process is linked to an enzyme, and in the final step of the process, to a substance with the enzyme’s substrate. The process will result to a subsequent reaction, which produces a detectable signal—typically a color change in the enzyme’s substrate that was added in the final step.
There are different types of enzyme-linked immunosorbent assay: the indirect, the sandwich, multiple and portable, and the competitive ELISA. These types of assay processes can be used for various purposes, and are typically available in kits that are relatively easy to use.
The ELISA can also be used to detect and evaluate the process of an antibody in the sample, which means that it can be used to determine serum antibody concentrations in tests for the HIV or the West Nile Virus. Food industries also rely in this test to detect potential allergens in items such as milk, peanuts, almonds, eggs, and walnuts.
This category contains scientific information of the enzyme-linked immunosorbent assay, a type of test that determines the antigen and antibody concentration in a certain sample.
Bhattarakosol P., 1987: Evaluation of biotin streptavidin elisa for detection of genital herpes simplex virus infection. Asian Pacific Journal Of Allergy & Immunology: 143-148 A biotin-streptavidin enzyme-linked immunosorbent assay (B-Sa Elisa) was evaluated for detection of herpes simplex virus (Hsv) in clinical specimens which were cervico-vaginal swabs from 205 asymptomatic women and swabs from the [...]
Mohan S.B., 1988: Evaluation of antisera raised against phytophthora fragariae for detecting the red core disease of strawberries by elisa. Plant Pathology (oxford): 206-216 Antiserum was raised against pooled mycelial suspensions from five isolates (designated Pf 1, Pf 2, Pf 3, Pf 10 and Pf 11) representing five physiologic races of Phytophthora fragariae. In enzyme-linked [...]
Schmitt M.E., 1985: Evaluation of an enzyme linked immunosorbent assay elisa for treponemal antibody. Journal Of Clinical Microbiology: 399-402 A new enzyme-linked immmunosorbent assay with Treponema pallidum antigen bound to ferrous metal beads (Syphilis Bio-EnzaBead; Litton Bionetics Laboratory Products) was compared with the standard fluorescent treponemal antibody-absorption test for syphilis. Bio-EnzaBead and fluorescent treponemal antibody-absorption [...]
Lumsden J.H., 1980: Evaluation of an enzyme linked immuno sorbent assay for determination of plasma thyroxine in dogs. Zentralblatt Fuer Veterinaermedizin Reihe A: 9-15 Plasma thyroxine (T4) content, pre- and post-Tsh stimulation, in euthyroid and primary hypothyroid dogs was simultaneously determined by an enzyme-linked immunosorbent assay (Elisa) and a radioimmunoassay (Ria). Using the Elisa technique [...]
Mcmillan A., 1982: Evaluation of an enzyme linked immuno sorbent assay for the detection of antibody to trichomonas vaginalis in sera and vaginal secretions. British Journal Of Venereal Diseases: 330-333 Using a whole-cell antigen, antibody to T. vaginalis was measured by an enzyme-linked immunosorbent assay (Elisa). IgG antibody was found in sera from only 3 [...]
De-Vries, G. P.; Stout, J. P. J.; Sanders, G. T. B., 1984: Evaluation of an enzyme linked immuno sorbent assay elisa for prostatic acid phosphatase ec 22.214.171.124. Clinica Chimica Acta 140(3): 279-288 An enzyme-linked immunosorbent assay (Elisa) was developed to measure prostatic acid phosphatase in human sera. The value of this method was tested on [...]
Lavin M.F., 1988: Evaluation of an enzyme immunoassay test for the diagnosis of chlamydia psittaci infection in free ranging koalas phascolarctos cinereus in southeastern queensland australia. Journal Of Wildlife Diseases: 259-263 The Ideia Chlamydia Test, a commercially available antigen-capture enzyme-linked immunosorbent assay (Elisa) test, based on a monoclonal antibody for the detection of chlamydia in [...]
Bellemans R., 1985: Evaluation of an elisa for the detection of campylobacter jejuni antibodies and comparison with a complement fixation test. Journal Of Microbiology: 321-332 An enzyme-linked immunosorbent assay (Elisa) was developed for the detection of total anti-Campylobacter immunoglobulins in human sera. In this assay disintegrated Campylobacter bacteria were used as the antigen. Absorption tests [...]
Malcolm A.D.B., 1985: Evaluation of an elisa enzyme linked immunosorbent assay system for determination of class specific antibodies to native and denatured dna in man. Annals Of The Rheumatic Diseases: 507-513 Enzyme-linked immunosorbent assays (Elisa) were set up for determination of plasma IgG and IgM antibodies to native (n) and denatured (d) Dna. Normal male [...]
Jones O.W., 1985: Evaluation of amniotic fluid alpha fetoprotein using an enzyme linked immunosorbent assay elisa. Obstetrics & Gynecology: 341-345 A simple, inexpensive enzyme-linked immunosorbent assay using commercially available supplies is described. An examination of amniotic fluid.alpha.-fetoprotein (Afp) values for 15-20 wk gestation is presented. The interassay coefficient of variation was 12% (N = 40) [...]