In the field of biochemistry, there are two types of electrophoresis: the nucleic acid electrophoresis and the electrophoresis of proteins.
Nucleic acid electrophoresis, or DNA electrophoresis, is an analytical technique that allows a scientist or researcher to separate DNA or RNA fragments according to their size and reactivity. The nucleic acids that are to be analyzed using this process are set upon a gel medium, where an electric field will be used to induce the nucleic acids toward the anode. This is made possible by the net negative charge of the backbone of the nucleic acid chain, which is made of sugar and phosphate molecules. The separation of the nucleic acid fragments is done through exploiting the mobilities, with which molecules of different sizes are able to pass through the viscous medium. Smaller fragments typically end up nearer to the anode, while longer molecules migrate more slowly because the gel provides resistance to these particles. After a certain amount of time, the electric current will be removed and the resulting fragmentation gradient is analyzed.
Protein electrophoresis, on the other hand, uses a fluid or an extract in order to analyze the proteins contained within. Typically, a protein electrophoresis is performed with a small amount of the sample used in alternative ways—either with or without a supporting medium. There are different kinds of protein electrophoresis, including SDS polyacrylamide gel electrophoresis, electrofocusing, free flow electrophoresis, isotachophoresis, affinity electrophoresis, immunoelectrophoresis, counterelectrophoresis, and capillary electrophoresis. Each of these methods has different variations, as well as individual advantages and limitations. However, protein electrophoresis is limited by practical limitations regarding its use as a preparative method. Protein electrophoresis is very useful in medicine in terms of analyzing the proteins present in the blood serum.
This category contains scientific information on electrophoresis, a process used in biochemistry to determine and evaluate proteins or enzymes within a specific sample.
Williamson F.B., 1984: Flavobacterium heparinum 2 o sulfatase for 2 o sulfato delta 4 5 glycuronate terminated oligosaccharides from heparin. European Journal Of Biochemistry: 607-616 The glycosulfatase which hydrolyzes the 2-O-sulfate of the disaccharide, 4-deoxy-2-O-sulfato-.alpha.-L-threo-hex-4-enopyranosyl uronic acid-(1.fwdarw. 4)-2-deoxy-2-sulfamido-6-O-sulfato-D-glucose (.Delta.Ua-2s.fwdarw. GlcNS-6s), was isolated from the soluble fraction of disrupted Flavobacterium heparinum. The activity was purified 3300-fold [...]
Williamson F.B., 1987: Flavobacterium heparinum sulfamidase for d glucosamine sulfamate purification and characterization. European Journal Of Biochemistry: 633-638 A novel assay has been developed for 2-deoxy-2-sulphamido-D-glucose (GlcNS) sulphamidase from Flavobacterium heparinum. This has enabled the 1930-fold purificateion of the enzyme from a soluble fraction of bacterial homogenate. From Sds/polyacrylamide gel electrophoresis the enzyme was shown [...]
Hirasawa, M.; Tamura, G., 1984: Flavin and iron sulfur containing ferredoxin linked glutamate synthase ec 22.214.171.124 from spinach leaves. Journal of Biochemistry (Tokyo) 95(4): 983-994 Ferredoxin-dependent glutamate synthase (native enzyme) of spinach was purified to homogeneity in the presence of 2-oxoglutarate and NaCl and the properties of the enzyme were studied. The Mw of the [...]
Kreuzaler, F.; Ragg, H.; Heller, W.; Tesch, R.; Witt, I.; Hammer, D.; Hahlbrock, K., 1979: Flavanone synthase ec 126.96.36.199 from petroselinum hortense molecular weight subunit composition size of messenger rna and absence of pantetheinyl residue. European Journal of Biochemistry 99(1): 89-96 Flavanone synthase from irradiated cell suspension cultures of parsley was purified to apparent homogeneity. [...]
“Jorstad K.E., 1987: Fine spotted brown trout salmo trutta its phenotypic description and biochemical genetic variation. Canadian Journal Of Fisheries & Aquatic Sciences: 1775-1779 Initial sudies on a small population of brown trout (Salmo tutta) in the Hardangervidda area in Norway revealed specimens with remarkable morphological characteristics. About one third of the population was classified [...]
Zaini Rahman M., 1981: Filarial enzymes by the horizontal starch gel electrophoresis technique. Southeast Asian Journal Of Tropical Medicine & Public Health: 513-517 In preliminary studies, on adult filarial parasites, difference in the electrophoretic pattern of glucose phosphate isomerase (Gpi) of Brugia malayi, B. pahangi and the rat filarial worm, Breinlia booliati, was demonstrated. There [...]
Furk C., 1979: Field collections of aphis fabae homoptera aphididae studied by starch gel electrophoresis and iso electric focusing. Comparative Biochemistry & Physiology B: 225-230 Electrophoresis separations of 2 enzyme systems and isoelectric focusing of soluble proteins of black bean aphids (A. fabae Scop.) were examined. Results from the 2 systems supported each other and [...]
Wadstrom T., 1983: Fibronectin receptors from staphylococcus aureus. Journal Of Biological Chemistry: 3396-3401 A further characterization of the interaction of fibronectin with staphylococci is presented. The binding of 125i-fibronectin to S. aureus, strain Cowan 1, is specific, time-dependent, functionally irreversible and occurs to live and heat-killed cells. Staphylococci may be saturated with fibronectin at a [...]
Schirrmacher V., 1988: Fibrin autography of plasminogen activator by electrophoretic transfer into fibrin agar gels. Analytical Biochemistry: 411-416 An electrophoretic modification of the conventional fibrin autography that can be used for the detection of plasminogen activators (urokinase type and tissue type) and fibrin-degrading enzymes in complex biological fluids is described. After separation by sodium dodecyl [...]
Alter B.P., 1984: Fetal hemo globin synthesis in erythroid cultures in hereditary persistence of fetal hemo globin and beta 0 thalassemia. Blood: 1278-1284 To determine whether Hb regulation is normal in diseases affecting.beta.-globin gene expression, globin synthesis was examined in members of a family of a patient with hereditary persistence of fetal Hb/.beta.degree.-thalassemia (Hpfh/.beta.degree.-thal). The [...]