The term chromatography refers a set of laboratory procedures used in the separation of mixtures. Chromatography can be especially useful in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product. There are two most widely used methods of viral processing: viral removal and viral inactivation. Viral removal is a method where all the viruses are simply removed from a specific sample completely. The second method typically allows some viruses to remain in the final product, but alters the viruses to become non-infective.
Some of the common viruses removed by viral removal and viral inactivation are HIV-1 and HIV2, hepatitis A, B, and, C, and various parvoviruses. There are some cases, however, where the virus itself is the desired product of the process, as in the case of HIV research. In many cases, researchers are trying to extract the viruses from the blood samples for study, but not specifically for blood purification. These viral processing techniques are also commonly used to remove particles produced as a result of a viral infection.
Chromatography is especially useful in removing viruses, as well as purifying the protein present in the samples. Chromatographic techniques are also effective against all types of viruses, but the level of the removal of the virus is typically dependent on the column composition and the reagents used in the process. The effectiveness of chromatographic processes can vary greatly between different types of viruses, and this efficiency can change based on the buffer used in the process. One of the most common concerns in chromatographic processes in viral processing is the sanitation between batches whenever these processes are performed.
This category contains scientific information on chromatography in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product.
Bidlingmeyer B., 1986: Pre chromatographic derivatization of primary and secondary amines with a polymeric anhydride for improved high performance liquid chromatographic detection. Journal Of Chromatography: 335-344 A polymeric anhydride containing o-acetylsalicyl as the labelling moiety was utilized as a derivatization reagent in conjunction with high-performance liquid chromatography for primary and secondary amines. The derivatization reactions [...]
Pellizzari E.D., 1984: Post column addition of buffer for thermo spray liquid chromatography mass spectrometry identification of pesticides/. Analytical Chemistry: 1507-1514 Coaxial and right-angle tees were evaluated as methods of postcolumn buffer addition for thermospray Lc/Ms analysis. The coaxial tee, which showed slightly better total ion current stability, was optimized to produce the best sensitivity. [...]
Molenaar P.C., 1981: Possible cholinergic mechanisms in fertilization of xenopus laevis eggs. Proceedings Of The Royal Society Of London Series B Biological Sciences190: 59-72 The hypothesis that cholinergic mechanisms play a role in fertilization was tested in laevis. Acetylcholine (ACh) analyses were done with gas chromatography-mass spectrometry. Eggs and testis-derived sperm contained material identified as [...]
Levina E.V., 1984: Polyhydroxylated steroids of the starfish patiria pectinifera. Khimiya Prirodnykh Soedinenii: 8-741 The following polyhydroxysteroids were isolated from P. pectinifera and described: 5.alpha.-cholestan-3.beta.,6.alpha.,8,15.alpha.,16.beta.,26-hexaol and 6.alpha.-sulfohydroxy-5.alpha.-cholestan-3.beta.,4.beta.,7.alpha.,8,15.beta.,16.beta.,26-heptaol sodium salt. The steroid polyol sulfate was found in the starfish for the 1st time. The structure and physicochemical properties of the compounds were determined using Nmr 13c [...]
Montag A., 1988: Polycyclic aromatic hydrocarbons in native vegetable oils. Fett Wissenschaft Technologie: 163-167 18 Polycyclic aromatic hydrocarbons have been isolated from different native vegetable oils by liquid-liquid-extraction, column chromatography on silica gel and Gpc on Biobeads S-X The PAHs were identified by capillary gas chromatography-mass spectrometry (Mis). High Pah-contamination has been detected in olive [...]
Murthy K.R., 1987: Polycyclic aromatic hydrocarbons in c 8 isomer aromatic feed analysis by gc gc ms and gc ftir techniques. Journal Of Chromatographic Science: 237-246 Polycyclic aromatic hydrocarbons (PAHs), apart from their carcinogenic and mutagenic nature, create many problems in the petrochemical industry due to their tendency toward carbonization. Compounds in C8 aromatic isomer [...]
Mehkeri K.A., 1985: Polyclinic aromatic hydrocarbons in barbecue briquets. Journal Of Food Safety: 177-202 Nine different brands of barbecue briquets currently available in the Canadian consumer market were examined for the presence of polycyclic aromatic hydrocarbons (Pah). The total concentrations of Pah ranged between 2.5 to 13.0.mu.g per gram of briquet sample. Some 30 to [...]
Bush B., 1981: Poly nuclear aromatic hydro carbons in diesel emission particulates. Environment International: 229-234 A preliminary characterization of polynuclear aromatic hydrocarbons (Pah) in dichloromethane extracts of diesel exhaust particulates was described. Acid-base liquid-liquid partitioning followed by adsorption chromatography was used to isolate the Pah fraction. Compounds were identified primarily by the mass spectra of [...]
Wakeham, S. G.; Schaffner, C.; Giger, W., 1980: Poly cyclic aromatic hydro carbons in recent lake sediments compounds having anthropogenic origins. Geochimica et Cosmochimica Acta 44(3): 403-414 Polycyclic aromatic hydrocarbons (Pah) in sediment cores from Lake Lucerne, Lake Zurich and Greifensee, Switzerland, and Lake Washington, northwest Usa, were isolated, identified and quantified by glass capillary [...]
Gizzi, F.; Reginato, R.; Benfenati, E.; Fanelli, R., 1982: Poly chlorinated di benzo p dioxins and poly chlorinated di benzo furans in emissions from an urban incinerator average and peak values. Chemosphere 11(6): 577-584 Gas chromatography-low resolution mass spectrometry was used to quantitate Pcdd and Pcdf emitted from an urban incinerator. The amounts were determined [...]