The term chromatography refers a set of laboratory procedures used in the separation of mixtures. Chromatography can be especially useful in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product. There are two most widely used methods of viral processing: viral removal and viral inactivation. Viral removal is a method where all the viruses are simply removed from a specific sample completely. The second method typically allows some viruses to remain in the final product, but alters the viruses to become non-infective.
Some of the common viruses removed by viral removal and viral inactivation are HIV-1 and HIV2, hepatitis A, B, and, C, and various parvoviruses. There are some cases, however, where the virus itself is the desired product of the process, as in the case of HIV research. In many cases, researchers are trying to extract the viruses from the blood samples for study, but not specifically for blood purification. These viral processing techniques are also commonly used to remove particles produced as a result of a viral infection.
Chromatography is especially useful in removing viruses, as well as purifying the protein present in the samples. Chromatographic techniques are also effective against all types of viruses, but the level of the removal of the virus is typically dependent on the column composition and the reagents used in the process. The effectiveness of chromatographic processes can vary greatly between different types of viruses, and this efficiency can change based on the buffer used in the process. One of the most common concerns in chromatographic processes in viral processing is the sanitation between batches whenever these processes are performed.
This category contains scientific information on chromatography in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product.
Leffler H., 1983: Preparative separation of sialic acid containing lipids from sulfate group containing glyco lipids from small intestine of different animals analysis by thin layer chromatography and detection of novel species. Journal Of Biochemistry (tokyo): 1473-1486 Acidic glycolipids (gangliosides and glycolipid sulfates) were purified from nonglycolipid contaminants by silicic acid chromatography of their acetylated [...]
Emken E.A., 1981: Preparation of deuterium labeled methyl cis 9 cis 12 octadecadienoate. Chemistry & Physics Of Lipids: 3-10 An 8-step synthesis is described which gives an overall yield of.apprx. 30% methyl cis-9-cis-12-octadecadienoate-16,16,17,17-d The preparation utilizes easily obtainable starting materials. Tris(triphenylphosphine)chlororhodium catalyst is used for incorporation of the deuterium isotopes. The double bond in the [...]
Dressler R.L., 1980: Preparation of a radio brominated fluorescein for liver scanning. Journal Of Labelled Compounds & Radiopharmaceuticals: 73-80 A radiobrominated compound, 4,5,6,7-tetrachloro-2′,4′,5′,7′-tetrabromofluorescein-77Br4 was prepared from resorcinol, tetrachlorophthallic anhydride and Na77Br by a 2-step synthesis. The precursor was identified by spectrometry. Final purification utilized column chromatography. The product was identified by Tlc and spectrometry. The [...]
Marai L., 1979: Preparation and mass spectral behavior of some 5 beta cholenoic acids. Canadian Journal Of Biochemistry: 216-225 Monounsaturated 5.beta.-cholanoic acids with double bonds in rings A, B and C were prepared by POCl3 and ZnCl2 dehydration from natural bile acids with selectively blocked hydroxyl functions. The yields ranged from 15-100%. The products were [...]
Fedoronko M., 1985: Preparation and identification of aldolization products of 1 3 dimethoxy 2 propanone. Chemical Papers: 763-768 4-Hydroxy-1,3,5-trimethoxy-4-methoxymethyl-2-pentanone (Ii), 4,6-dihydroxy-1,3,5,7-tetramethoxy-4,6-bis(methoxymethyl)-2-heptanone (Iiia), and 4,6,8-trihydroxy-1,3,5,7,9-pentamethoxy-4,6,8-tris(methoxymethyl)-2-nonanone have been prepared by aldolization of 1,3-dimethoxy-2-propanone in aqueous NaOH solution at 5.degree. C. The components of the reaction mixture were separated by liquid chromatography and their structures were established by [...]
Cook R., 1983: Preparation and chemical composition of orange oil concentrates. Journal Of Food Science: 1197-1199 Flavor and color concentrates were prepared by a vacuum distillation process from cold pressed ‘Valencia’ and midseason orange oils and were analyzed qualitatively by gas chromatography. Major and minor constituents (25) were identified by mass spectrometry. The influence of [...]
Levison B., 1980: Preparation and characterization of the methoxy derivatives of poly unsaturated fatty acids stabilized product analogs of lip oxygenase catalysis. Lipids: 1051-1054 The products from the action of soybean lipoxygenase on a series of polyunsaturated fatty acids were converted in 2 steps into the corresponding methoxy derivatives. The product hydroperoxides were reduced in [...]
Dinga, A.; Braut-Boucher, F.; Paris, M.; Mercier, J., 1980: Preliminary study of labiatae originating from africa comparative analysis of essential oils of leocus africanus and alvesia rosmarinifolia. Herba Hungarica 19(1): 139-150 A preliminary study of the essential oils of L. africanus and A. rosmarinifolia revealed the importance of sesquiterpenes in the air-dried plants. Using column [...]
Masson C., 1984: Preliminary results about chemicals emitted by bumblebees bombus hypnorum hymenoptera apoidea bombinae. Comptes Rendus De L’Academie Des Sciences Serie Iii Sciences De La Vie: 297-302 Extracts of cuticles and labial glands of B. hypnorum were analyzed by coupled capillary chromatography and mass spectrometry. The ranges of terpenes and fatty acids derivatives are [...]
Bidlingmeyer B., 1986: Pre chromatographic derivatization of primary and secondary amines with a polymeric anhydride for improved high performance liquid chromatographic detection. Journal Of Chromatography: 335-344 A polymeric anhydride containing o-acetylsalicyl as the labelling moiety was utilized as a derivatization reagent in conjunction with high-performance liquid chromatography for primary and secondary amines. The derivatization reactions [...]