The term chromatography refers a set of laboratory procedures used in the separation of mixtures. Chromatography can be especially useful in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product. There are two most widely used methods of viral processing: viral removal and viral inactivation. Viral removal is a method where all the viruses are simply removed from a specific sample completely. The second method typically allows some viruses to remain in the final product, but alters the viruses to become non-infective.
Some of the common viruses removed by viral removal and viral inactivation are HIV-1 and HIV2, hepatitis A, B, and, C, and various parvoviruses. There are some cases, however, where the virus itself is the desired product of the process, as in the case of HIV research. In many cases, researchers are trying to extract the viruses from the blood samples for study, but not specifically for blood purification. These viral processing techniques are also commonly used to remove particles produced as a result of a viral infection.
Chromatography is especially useful in removing viruses, as well as purifying the protein present in the samples. Chromatographic techniques are also effective against all types of viruses, but the level of the removal of the virus is typically dependent on the column composition and the reagents used in the process. The effectiveness of chromatographic processes can vary greatly between different types of viruses, and this efficiency can change based on the buffer used in the process. One of the most common concerns in chromatographic processes in viral processing is the sanitation between batches whenever these processes are performed.
This category contains scientific information on chromatography in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product.
Fujii S., 1988: Platelet aggregation inhibitors from jyu yaku houttuyniae herb. Chemical & Pharmaceutical Bulletin (tokyo): -1904 Two potent inhibitors of platelet aggregation were isolated from the chloroform extract of jyuyaku (Houttuyniae herb), a traditional medicine in Japan and China by a combination of high-performance liquid chromatography and other techniques. These compounds were identified as [...]
Saito K., 1986: Platelet activating factor in normal rat uterus. Biochemical & Biophysical Research Communications: 1231-1236 Platelet-activating factor (Paf) was found in normal rat uterus and identified as 1-O-hexadecyl/octadecenyl-2-acetyl-sn-glycero-3-phosphocholine. Paf was purified by several successive chromatographic procedures. Cv 3988, and had no effect on platelets desensitized with 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine. The tert-butyldimethylsilylderivative of 1-O-alkyl-2-acetyl-sn-glycerol, which was obtained [...]
Pickett W.C., 1986: Platelet activating factor and leukotriene biosynthesis is inhibited in polymorphonuclear leukocytes depleted of arachidonic acid. Journal Of Biological Chemistry7: 7592-7595 Rat peripheral or elicited polymorphonuclear leukocytes 90% deficient in arachidonic acid incorporate, after stimulation with the calcium ionophore A23187, 86% less acetate into platelet-activating factor than control. The total amount of platelet-activating [...]
Steiner J.A., 1986: Plasma levels of nitroglycerin generated by three nitroglycerin patch preparations nitradisc transiderm nitro and nitro dur and one ointment formulation nitrobid. British Journal Of Clinical Pharmacology: 365-370 Twenty-four healthy male subjects participated in a study comparing plasma concentrations of nitroglycerin generated by single applications of Nitradisc 32 mg, Transiderm-Nitro 50 mg and [...]
Laumas K.R., 1979: Plasma levels of 15s 15 methyl prostaglandin f 2 alpha methyl ester following vaginal administration for induction of abortion in women. Contraception: 519-531 A single suppository containing 3.0 mg 15 (S)15-methyl-prostaglandinF2.alpha.-methyl ester was administered vaginally in women to terminate mid-trimester pregnancy. Plasma levels of the drug (both methyl ester and free acid [...]
Makin H.L.J., 1980: Plasma concentrations of alphaxalone by gas chromatography comparison with other gas chromatographic methods and gas chromatography mass spectrometry. Journal Of Pharmacy & Pharmacology: 349-352 Existing methods for the measurement of plasma or tissue alphaxalone (3.alpha.-hydroxy-5.alpha.-pregnane-11,20 dione) concentrations by gas chromatography are not wholly satisfactory because of the problems of interfering peaks and [...]
Lawson A.M., 1987: Plasma bile acids in patients with peroxisomal dysfunction syndromes analysis by capillary gas chromatography mass spectrometry. European Journal Of Pediatrics: 166-173 Six patients with disorders of peroxisomal function have been studied. Two presented in the neonatal period with the classical features of the Zellweger syndrome, two had incomplete Zellweger phenotypes, one infantile [...]
Kato J., 1986: Plant growth regulators in cucumis melo var flexuosus fruit during rapid growth. Plant & Cell Physiology: 809-818 The fruit growth of the snake melon (Cucumis melo L. var. flexuosus Naud) and the plant hormones contained in its immature fruit were investigated. The fruit growth started 5 days after pollination and its rapid [...]
Bier D.M., 1981: Picomole assay for n tau methyl histidine by gas chromatography mass spectrometry. Analytical Biochemistry: 308-317 A simple, sensitive method for measuring N.tau.-methylhistidine in biological samples using a deuterated internal standard and methane chemical ionization gas chromatography-mass spectrometry is described. After sample preparation a single analysis can be completed in 3 min; analysis [...]
Saatov, Z.; Gorovits, M. B.; Abdullaev, N. D.; Abubakirov, N. K., 1987: Phytoecdysteroids from plants of the genus silene xii. 5 alpha ecdysterone 22 o benzoate from silene scabriofolia. Khimiya Prirodnykh Soedinenii (5): 678-680 A new ecdysteroid was isolated from the aboveground organs of S. scabrifolia, using column chromatography. The structure of 5.alpha.-ecdysterone-22-0-benzoate was established [...]