The term chromatography refers a set of laboratory procedures used in the separation of mixtures. Chromatography can be especially useful in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product. There are two most widely used methods of viral processing: viral removal and viral inactivation. Viral removal is a method where all the viruses are simply removed from a specific sample completely. The second method typically allows some viruses to remain in the final product, but alters the viruses to become non-infective.
Some of the common viruses removed by viral removal and viral inactivation are HIV-1 and HIV2, hepatitis A, B, and, C, and various parvoviruses. There are some cases, however, where the virus itself is the desired product of the process, as in the case of HIV research. In many cases, researchers are trying to extract the viruses from the blood samples for study, but not specifically for blood purification. These viral processing techniques are also commonly used to remove particles produced as a result of a viral infection.
Chromatography is especially useful in removing viruses, as well as purifying the protein present in the samples. Chromatographic techniques are also effective against all types of viruses, but the level of the removal of the virus is typically dependent on the column composition and the reagents used in the process. The effectiveness of chromatographic processes can vary greatly between different types of viruses, and this efficiency can change based on the buffer used in the process. One of the most common concerns in chromatographic processes in viral processing is the sanitation between batches whenever these processes are performed.
This category contains scientific information on chromatography in viral processing, which aims to stop the viruses in a specific sample from infecting the desired product.
Korhammer, Sa; Haslinger, E., 1994: Isolation of a biologically active substance from rhizomes of quackgrass. Journal of agricultural and food chemistry 42(9): 2048-2050 Quackgrass seems to possess allelopathic properties. The substances responsible for this are still unknown. A substance having strong inhibitory effects on the germination of seeds of alfalfa at concentrations lower than 10(-6) [...]
Nielsen, Jacob H.; Olsen, Carl Erik; Duedahl, Christina; Skibsted, Leif H., 1995: Isolation and quantification of cholesterol oxides in dairy products by selected ion monitoring mass spectrometry. Journal Of Dairy Research. 62(1): 101-113 A method for isolation, detection and quantification of cholesterol oxidation products based on solid phase extraction in combination with preparative Hplc and [...]
Vetter, Walter; Luckas, Bernd; Oehme, Michael, 1992: Isolation and purification of the two main toxaphene congeners in marine organisms. Chemosphere. 25(11): 1643-1652 The two most abundant toxaphene congeners in marine mammals and fish were isolated from 3,5 kg seal blubber. In a first step the tissue was decomposed by perchloric/acetic acid followed by extraction with [...]
Oden, Per Christer; Brandtberg, Per Olov; Andersson, Rolf; Gref, Rolf; Zackrisson, Olle; Nilsson, Marie Charlotte, 1992: Isolation and characterization of a germination inhibitor from leaves of Empetrum hermaphroditum Hagerup. Scandinavian Journal Of Forest Research. 7(4): 497-502 An extract of Empetrum hermaphroditum Hagerup leaves was examined for germination inhibitors using an assay based on germination of [...]
James, D. G.; Moore, C. J.; Aldrich, J. R., 1994: Identification, synthesis, and bioactivity of a male-produced aggregation pheromone in assassin bug, Pristhesancus plagipennis (Hemiptera: Reduviidae). Journal of Chemical Ecology 20(12): 3281-3295 Pristhesancus plagipennis, a large Australian assassin bug, possesses three pairs of dorsal abdominal glands (DAGs). In the male, the anterior and posterior glands [...]
Umano, K.; Hagi, Y.; Tamura, T.; Shoji, A.; Shibamoto, T., 1994: Identification of volatile compounds isolated from round kumquat (Fortunella japonica Swingle). Journal of Agricultural and Food Chemistry 42(9): 1888-1890 Volatile constitutents of round kumquat fruit were isolated by steam distillation and simultaneous purging/extraction (Spe) methods. The isolated volatiles were identified by gas chromatography and [...]
Battistini, M.; Griffini, A.; Peterlongo, F., 1995: Identification of tanshinones from Salvia miltiorrhiza roots by liquid chromatography-thermospray mass spectrometry. Fitoterapia. 66(3): 265-272 Liquid chromatography-thermospray mass spectrometry (Lc-Tsp Ms) was used to identify tanshinones in S. miltiorrhiza root methanolic extract.
Oka, Hisao; Ikai, Yoshitomo; Hayakawa, Junko; Harada, Ken Ichi; Masuda, Katsuyoshi; Suzuki, Makoto; Himei, Ruriko; Horie, Masakazu; Nakazawa, Hiroyuki, 1993: Identification of residual tetracyclines in honey by TLC/FABMS. Journal Of The Food Hygienic Society Of Japan. 34(6): 517-523 In order to reliably identify residual tetracycline antibiotics (TCs), oxytetracycline, tetracycline, chlortetracycline, and doxycycline, in honey, a [...]
Mehta, Am; Saftner, Ra; Mehta, Ra; Davies, Pj, 1994: Identification of posttranslationally modified 18-kilodalton protein from rice as eukaryotic translation initiation factor 5A. Plant physiology 106(4): 1413-1419 Using anther-derived rice (Oryza sativa L.) cell-suspension cultures, we have identified an 18-kD protein that is posttranslationally modified by spermidine and is influenced by endogenous polyamine levels. The [...]
Dougherty, M. J.; Guerin, P. M.; Ward, R. D., 1995: Identification of oviposition attractants for the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) in volatiles of faeces from vertebrates. Physiological Entomology 20(1): 23-32 Extracts of volatiles from rabbit and chicken faeces preferentially attracted gravid sandflies, Lutzomyia longipalpis (Lutz and Neiva), in an oviposition bioassay. In electrophysiology experiments, [...]