Sepharose is the tradename for a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed. When used in research techniques (such as various forms of chromatography), iodoacetyl functional groups can be added to selectively bind the cysteine side chains in the material. This method of adding iodoacetyl functional groups is often used in procedures that require the immobilization of peptides. This brand name of polysaccharide polymer is derived from the name “Separation Pharmacia Agarose.” Sepharose is commonly used in chromatographic separations of a wide variety of biomolecules.
Sepharose is a registered trademark that belongs to GE Healthcare (the company formed from the former Pharmacia, Pharmacia Biotech, Pharmacia LKB Biotechnology, Amersham Biosciences, and Amersham Pharmacia Biotech). In the late 1950’s, Pharmacia launched Sephadex, a cross-linked dextran gel used for gel filtration. For decades, the company has continued to spearhead advances in the field of chromatography through the introduction of Sepharose in 1967.
Sepharose is known for its chemical versatility, which allows for the stable attachment of ligands for the purification of a wide variety of enzymes, antibodies, peptides, and other proteins. Because of its versatility and high mechanical stability, Sepharose is an excellent base matrix for many high performance chromatographic procedures in affinity chromatography, ion exchange chromatography, and other processes of separation.
Coupled with a particular form of activation chemistry, sepharose can also be used to immobilize many types of enzymes, antibodies, and other kinds of proteins and peptides through covalent attachment to the resin. Typical types of activation chemistry processes include the reductive animation of aldehydes (in order to attach proteins to the resin in the agarose through lysine side chains) and cyanogen bromide.
This category contains scientific information on Sepharose, a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed, produced and distributed by GE Healthcare.
Marshall A.G., 1984: Preparative scale isolation and purification of prokaryotic and eukaryotic ribosomal 5s rna bacillus subtilis neurospora crassa and wheat germ/. Analytical Biochemistry: 465-471 Ribosomal 5 S Rna from 3 different organisms was isolated in high yield and purity. Without prior isolation of ribosomes, a presoak in buffer followed by phenol extraction, De-32 ion-exchange [...]
Viale A.M., 1984: Preparative purification of the subunits of chloroplast and rhodospirillum rubrum coupling factors by flat bed electrofocusing in granulated gels. Journal Of Biochemical & Biophysical Methods2: 103-110 A rapid method for the preparative purification of the subunits of oligomeric proteins like spinach chloroplast and Rhodospirillum rubrum coupling factors is presented. It involves the [...]
Schrade H., 1984: Preparative isolation of tetra purine penta purine and hexa purine oligo nucleotides from partial hydrolysates of depyrimidinated herring sperm dna. Journal Of Chromatography: 381-408 Herring sperm Dna is chemically degraded to a complex mixture of purine nucleotides. The oligonucleotides are separated from the partial hydrolysates by column chromatography. The resulting mixture of [...]
“Schott H., 1987: Preparative isolation of purine oligonucleotides from partial hydrolysates of depyrimidinated dna. Separation Science & Technology10: 2061-2100 Dna is chemically degraded to a mixture of pure oligonucleotides. From the resulting partial hydrolyzate, defined oligonucleotides with two to six monomer units and defined mixtures of sequence-isomeric purine oligonucleotides are isolated in preparative amounts. The [...]
Watzlawick H., 1982: Preparative isolation of oligo guanosine phosphates from partial hydrolyzates of dna using template chromatography. Journal Of Chromatography: 57-70 Dna from herring sperm is partially hydrolyzed to oligoguanosine phosphates. High-molecular-weight fragments are isolated from the partial hydrolyzate using Deae-cellulose and afterwards Qae-Sephadex. The mixture of guanosine oligonucleotides is separated into complementary and non-complementary [...]
Schrade H., 1983: Preparative isolation of mono purine di purine and tri purine nucleotides from hydrolyzates of de pyrimidinated herring sperm dna. Journal Of Chromatography: 257-276 The purine nucleotides pdAp, pdGp, (dA)2, (dA-dG), (dG-dA), (dG)2, (dA)3, (dA-dG-dA), (dA-dA-dG), (dG-dA-dA), (dG-dA-dG) and known mixtures of purine nucleotide sequence isomers were separated by preparative scale chromatography of [...]
Schott H., 1984: Preparative isolation of hexa pyrimidine hepta pyrimidine octa pyrimidine nona pyrimidine and deca pyrimidine oligo nucleotides from hydrolysates of depurinated herring sperm dna. Journal Of Chromatography: 409-432 The pyrimidine oligonucleotides (dT)5, (dT)6 and the mixtures of sequence isomers (dC, dT5); (dC2, dT5); (dC3, dT4); (dC4, dT3); (dC4, dT4); (dC3, dT5); (dC2, dT6); [...]
Pham T.D., 1987: Preparative electrophoresis on agarose submerged gels of two aggregating proteoglycan monomers from articular cartilage. Preparative Biochemistry: 229-238 Analytical electrophoresis on polyacrylamide-agarose gels of aggregating proteoglycan monomers from baboon articular cartilage produces two distinct bands2-5, corresponding to two different aggregating monomer populations3, A preparative electrophoresis procedure is described for isolating the two monomers. [...]
Petrovic J., 1982: Preparation of technetium 99 di methyl sulfoxide renal complex in solution and its chemical and biological characterization. International Journal Of Applied Radiation & Isotopes: 277-284 99Tc-Dms solution was prepared by double isotope labeling, purified by molecular sieving through Sepharose 2b column, and checked by chromatographic and spectrophotometric methods, as well as by [...]
Kull F.J., 1980: Preparation of oligo nucleotides and their use in molecular weight estimations. Preparative Biochemistry: 359-368 Yeast Rna was used to prepare oligonucleotides employed to calibrate a G-50 Sephadex column. The oligonucleotides’ preparation, isolation, desalting and characterization is described. Data obtained by chromatography of the oligonucleotides demonstrate that the Mw of oligonucleotides can be [...]