Sepharose is the tradename for a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed. When used in research techniques (such as various forms of chromatography), iodoacetyl functional groups can be added to selectively bind the cysteine side chains in the material. This method of adding iodoacetyl functional groups is often used in procedures that require the immobilization of peptides. This brand name of polysaccharide polymer is derived from the name “Separation Pharmacia Agarose.” Sepharose is commonly used in chromatographic separations of a wide variety of biomolecules.
Sepharose is a registered trademark that belongs to GE Healthcare (the company formed from the former Pharmacia, Pharmacia Biotech, Pharmacia LKB Biotechnology, Amersham Biosciences, and Amersham Pharmacia Biotech). In the late 1950’s, Pharmacia launched Sephadex, a cross-linked dextran gel used for gel filtration. For decades, the company has continued to spearhead advances in the field of chromatography through the introduction of Sepharose in 1967.
Sepharose is known for its chemical versatility, which allows for the stable attachment of ligands for the purification of a wide variety of enzymes, antibodies, peptides, and other proteins. Because of its versatility and high mechanical stability, Sepharose is an excellent base matrix for many high performance chromatographic procedures in affinity chromatography, ion exchange chromatography, and other processes of separation.
Coupled with a particular form of activation chemistry, sepharose can also be used to immobilize many types of enzymes, antibodies, and other kinds of proteins and peptides through covalent attachment to the resin. Typical types of activation chemistry processes include the reductive animation of aldehydes (in order to attach proteins to the resin in the agarose through lysine side chains) and cyanogen bromide.
This category contains scientific information on Sepharose, a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed, produced and distributed by GE Healthcare.
Erbe R.W., 1981: Folate poly glutamate and mono glutamate accumulation in normal and sv 40 transformed human fibroblasts. Journal Of Cellular Physiology: 497-506 Folate polyglutamate and monoglutamate accumulation was measured in normal diploid and Sv40-transformed human fibroblasts by Sephadex G-10 gel filtration chromatography. The cells were first depleted of folates and then provided with limiting [...]
Hirose S., 1988: Fluorometric determination of sisomicin an aminoglycoside antibiotic in dried blood spots on filter paper by reversed phase high performance liquid chromatography with pre column derivatization. Chemical & Pharmaceutical Bulletin (tokyo): 1571-1574 A simple method for the determination of sisomicin (Siso) in dried blood spots (Dbs) on filter paper has developed. Siso in [...]
Mccormick J.J., 1979: Fluorescent labeling of fragments of high molecular weight rna. Analytical Biochemistry: 399-406 A method to fluorescently label microgram quantities of high Mw Rna with acriflavine is described. The method involves hydrolyzing the Rna with HCl at pH 1.0 for 10 min to obtain segments of about 80 nucleotides. The 3′-terminal phosphate is [...]
Ingham K.C., 1983: Fluid phase interaction between human plasma fibronectin and gelatin determined by fluorescence polarization assay. Archives Of Biochemistry & Biophysics: 358-366 Previous studies of the binding properties of fibronectin (Fn) have utilized methods whereby one or the other macromolecule was immobilized on a solid phase. To examine the interaction between human plasma Fn [...]
Colomb M.G., 1982: Fluid phase activation of pro enzymic complement c 1r purified by affinity chromatography. Biochimica Et Biophysica Acta: 118-126 Proenzymic C1r was purified from human plasma in a 2-step technique involving indirect affinity chromatography on Sepharose Ig anti-C.hivin. The capacity of C1r to monomerize at pH 5.0 and to redimerize at neutral pH [...]
Grindley T.B., 1987: Flavonoid glycosides of spartan apple peel. Journal Of Agricultural & Food Chemistry: 529-531 The flavonoid glycosides of Spartan apples were isolated by column chromatography on polyamide and Sephadex resins and by Rp-Hplc. They were characterized by 1h and 13c Nmr as phlorizin and the following glycosides of quercetin:.alpha.-L-arabinofuranoside,.beta.-D-galactoypyranoside,.beta.-D-glucopyranoside,.alpha.-L-rhamnopyranoside,.beta.-D-xylopyranoside. The coupling constants in [...]
Targamadze I.L., 1986: Flavanones from citrus unshiu fruits. Prikladnaya Biokhimiya I Mikrobiologiya: 423-427 Two flavanones were isolated from the methanol extract of the Citrus unshiu Marc. fruit pulp by counter-current distribution and column chromatography on polyamide and Sephadex. By means of Uv, Ir, and Pmr spectroscopy they were identified as isosakuranetin-7-rutinoside (didimine) and naringenin-7-rutinoside (narirutine).
Fincher G.B., 1987: Fine structure of the arabinogalactan protein from lolium multiflorum. Carbohydrate Research: 85-94 The extracellular arabinogalactan-protein from suspension-cultured cells of Lolium multiflorum (ryegrass) was purified in a single step by affinity chromatography of the culture medium on myeloma protein J539-Sepharose. The product obtained after two Smith-degradations had an apparent molecular size corresponding to [...]
Goodwin J.L., 1988: Fibronectin receptors of phagocytes characterization of the arg gly asp binding proteins of human monocytes and polymorphonuclear leukocytes. Journal Of Experimental Medicine: 777-793 We have defined the cell surface molecules of human monocytes and Pmn that bind to the chymotryptic cell binding domain of Fn and to a synthetic peptide, Kyavtgrgds, based [...]