Sepharose is the tradename for a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed. When used in research techniques (such as various forms of chromatography), iodoacetyl functional groups can be added to selectively bind the cysteine side chains in the material. This method of adding iodoacetyl functional groups is often used in procedures that require the immobilization of peptides. This brand name of polysaccharide polymer is derived from the name “Separation Pharmacia Agarose.” Sepharose is commonly used in chromatographic separations of a wide variety of biomolecules.
Sepharose is a registered trademark that belongs to GE Healthcare (the company formed from the former Pharmacia, Pharmacia Biotech, Pharmacia LKB Biotechnology, Amersham Biosciences, and Amersham Pharmacia Biotech). In the late 1950’s, Pharmacia launched Sephadex, a cross-linked dextran gel used for gel filtration. For decades, the company has continued to spearhead advances in the field of chromatography through the introduction of Sepharose in 1967.
Sepharose is known for its chemical versatility, which allows for the stable attachment of ligands for the purification of a wide variety of enzymes, antibodies, peptides, and other proteins. Because of its versatility and high mechanical stability, Sepharose is an excellent base matrix for many high performance chromatographic procedures in affinity chromatography, ion exchange chromatography, and other processes of separation.
Coupled with a particular form of activation chemistry, sepharose can also be used to immobilize many types of enzymes, antibodies, and other kinds of proteins and peptides through covalent attachment to the resin. Typical types of activation chemistry processes include the reductive animation of aldehydes (in order to attach proteins to the resin in the agarose through lysine side chains) and cyanogen bromide.
This category contains scientific information on Sepharose, a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed, produced and distributed by GE Healthcare.
Wongkham, Sopit; Wongkham, Chaisiri; Boonsiri, Patcharee; Simasathiansophon, Sontaya; Trisonthi, Chusri; Atisook, Kanit, 1995: Isolectins from seeds of Artocarpus lakoocha. Phytochemistry (oxford). 40(5): 1331-1334 Two isolectins (Ala-I and Ala-Ii), were isolated from seed extracts of Artocarpus lakoocha by anion exchange chromatography on Q-Sepharose fast flow columns at pH 8.5 and 8.0. Ala-I was unbound to the [...]
Sakai, Shingo; Nakajimi, Masatoshi; Yamaguchi, Isomaro, 1994: Isolation of gibberellin-binding proteins by affinity chromatography. Bioscience Biotechnology & Biochemistry. 58(5): 963-964 Gibberellin-binding proteins (GBPs) were isolated from a crude extract of etiolated maize seedlings by affinity chromatography on gibberellin A-3(Ga-3)-linked enhance-aminohexyl(Eah)-Sepharose 4b. The isolated proteins had a binding activity for (3h)-Ga- The binding site with dissociation [...]
Tsai, C. S.; Guede Guina, F.; Smith, M. O.; Vangah Manda, M.; Ochillo, R. F., 1995: Isolation of cholinergic active ingredients in aqueous extracts of Mareya micrantha using the longitudinal muscle of isolated guinea-pig ileum as a pharmacological activity marker. Journal Of Ethnopharmacology. 45(3): 215-222 In our attempt to isolate the pharmacologically active ingredients in [...]
Bento, A. C.; Rego, E.; Pedroso Mariani, S. R.; Mancuso, L. C.; Giglio, J. R.; Novello, J. C.; Marangoni, S.; Caracelli, I.; Oliveira, B.; Antunes, E.; De Nucci, G., 1995: Isolation of a polypeptide from Phoneutria nigriventer spider venom responsible for the increased vascular permeability in rabbit skin. Toxicon. 33(2): 171-178 Fractionation of Phoneutria nigriventer [...]
Mcgonigle, S.; Dalton, J. P., 1995: Isolation of Fasciola hepatica haemoglobin. Parasitology. 111(2): 209-215 A hemoprotein released in vitro by adult Fasciola hepatica was purified by gel filtration chromatography on Sephacryl S-200 and ion-exchange chromatography on Deae-Sepharose. The molecule, with an apparent molecular weight of gt 200 kDa, contains a haem group and has absorption [...]
Ralet, Marie Christine; Thibault, Jean Francois; Faulds, Craig B.; Williamson, Gary, 1994: Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp. Carbohydrate Research. 263(2): 227-241 Cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. Enzymic as well as chemical hydrolysis of the pulp yielded a series [...]
Liu, Dly; Christians, Ne, 1994: Isolation and identification of root-inhibiting compounds from corn gluten hydrolysate. Journal of plant growth regulation 13(4): 227-230 Interest has centered on the use of plant-derived compounds as natural herbicides, and they are considered to represent an environmentally sound approach to weed control. Corn gluten hydrolysate, found to have a growth-regulating [...]
Karmakar, Pr; Chatterjee, Bp, 1994: Isolation and characterization of two IgE-reactive proteins from Azadirachta indica pollen. Molecular and cellular biochemistry 131(1): 87-96 Two allergenically active components present in the Azadirachta indica whole pollen extract have been isolated by sequential ammonium sulfate precipitation (0-90%), Deae-Sephadex A-50 ion-exchange chromatography followed by gel filtration through Sephadex G-200. The [...]
Gong, Ming; Yang, Zhong Han; Tsao, T. H., 1993: Isolation and characterization of calmodulin and a novel calcium-binding protein calpollenin from Pinus yunnanensis pollen. Plant Science (limerick). 89(1): 5-12 A protein identifiable as calmodulin (CaM) was separated from Pinis yunnanensis pollen by phenyl-sepharose affinity chromatography and further purified by preparative polyacrylamide gel electrophoresis (Page). This [...]
Fukuda, Nobuhiro; Yoshimaru, Atsushi; Hidaka, Toshiro; Ohta, Hideaki; Yamamoto, Kyosuke; Yomo, Harugoro, 1994: Isolation and characterization of N-acetylgalactosamine-specific lectin from Galactia tashiroi seeds. Bioscience Biotechnology & Biochemistry. 58(2): 423-424 An N-acetylgalactosamine-specific lectin was isolated from Galactia tashiroi seeds by affinity chromatography on Galactose-Sepharose 6b. It was a glycoprotein with molecular weight of 90,000-95,000 and composed [...]