Sepharose is the tradename for a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed. When used in research techniques (such as various forms of chromatography), iodoacetyl functional groups can be added to selectively bind the cysteine side chains in the material. This method of adding iodoacetyl functional groups is often used in procedures that require the immobilization of peptides. This brand name of polysaccharide polymer is derived from the name “Separation Pharmacia Agarose.” Sepharose is commonly used in chromatographic separations of a wide variety of biomolecules.
Sepharose is a registered trademark that belongs to GE Healthcare (the company formed from the former Pharmacia, Pharmacia Biotech, Pharmacia LKB Biotechnology, Amersham Biosciences, and Amersham Pharmacia Biotech). In the late 1950’s, Pharmacia launched Sephadex, a cross-linked dextran gel used for gel filtration. For decades, the company has continued to spearhead advances in the field of chromatography through the introduction of Sepharose in 1967.
Sepharose is known for its chemical versatility, which allows for the stable attachment of ligands for the purification of a wide variety of enzymes, antibodies, peptides, and other proteins. Because of its versatility and high mechanical stability, Sepharose is an excellent base matrix for many high performance chromatographic procedures in affinity chromatography, ion exchange chromatography, and other processes of separation.
Coupled with a particular form of activation chemistry, sepharose can also be used to immobilize many types of enzymes, antibodies, and other kinds of proteins and peptides through covalent attachment to the resin. Typical types of activation chemistry processes include the reductive animation of aldehydes (in order to attach proteins to the resin in the agarose through lysine side chains) and cyanogen bromide.
This category contains scientific information on Sepharose, a cross-linked and beaded form of a polysaccharide polymer, which is extracted from seaweed, produced and distributed by GE Healthcare.
Leduc D., 1984: Preliminary characterization of inhibitors of neisseria gonorrhoeae produced in vitro by eubacterium limosum. Canadian Journal Of Microbiology: 251-259 Among anaerobic bacteria normally found in the urogenital flora, E. limosum inhibited the in vitro growth of N. gonorrhoeae. The antigonococcal activity produced by E. limosum was soluble in methanol and in a CHCl3-methanol [...]
Gaede G., 1984: Preliminary characterization of glycogen phosphorylase activating hormone and adipo kinetic hormone from manduca sexta corpora cardiaca. Physiological Entomology: 229-236 An attempt was made to separate glycogen phosphorylase activating hormone (Gpah) and adipokinetic hormone (Akh) from the corpora cardiaca (Cc) of M. sexta (Lepidoptera: Sphingidae) by separating extracts of Cc on various chromatographic [...]
Levy J.G., 1985: Preliminary characterization of a soluble immunosuppressive molecule from dba 2 spleen cells using monoclonal antibody immunoadsorbance. Cellular Immunology: 303-313 In a previous publication a monoclonal antibody (B16g) which appeared to recognize T suppressor cells and a T-suppressor factor (TsF) in the spleens of Dba/2 mice was described. B16g appears to be directed [...]
Gianni, A. M.; Presta, M.; Polli, E.; Peschle, C.; Lettieri, F.; Saglio, G.; Comi, P.; Giglioni, B.; Ottolenghi, S., 1982: Preferential induction of fetal vs. embryonic globin chains in human leukemic cell lines. Leukemia Research 6(2): 155-164 By use of a newly developed technique combining affinity chromatography of Hb on haptoglobin-Sepharose and Ief of globin [...]
Mirocha C.J., 1979: Preferential binding of radio labeled zearalenone to a protein fraction of fusarium roseum graminearum. Applied & Environmental Microbiology: 80-84 Zearalenone is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3h- and 14c-labeled zearalenone bound preferentially to 1 of 2 peaks containing uncharacterized proteins obtained from the [...]
Ueda K., 1986: Preferential association of acidic actin with nuclei and nuclear matrix from mouse leukemia l 5178y cells. Experimental Cell Research: 327-336 Nuclear matrix prepared from mouse leukemia L5178y cells contained not only the two common actin isomers, beta and gamma actins, but also two additional acidic species of actin (pI 5.1 and 5.3). [...]
Pickering B.T., 1982: Precursors in the biosynthesis of vasopressin and oxytocin in the rat characteristics of all the components in high performance liquid chromatography. Biochemical Journal: 339-350 A reverse-phase high performance liquid-chromatography (Hplc) protocol was developed, whereby all the major known posterior-pituitary components that are derived from the processing of pro-oxytocin and pro-vasopressin can be [...]
Pizzo S.V., 1985: Precipitation of fibrinogen fibrinogen degradation products and fibrin monomer by histone h 3. Thrombosis Research: 97-116 Incubation of histone H3 with normal citrated plasma resulted in the formation of insoluble aggregates, as determined by turbidity measurements. The precipitate was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis, confirming that fibrinogen was a [...]
Okuno A., 1982: Pre natal diagnosis of meta chromatic leuko dystrophy a diagnosis by amniotic fluid and its confirmation. Archives Of Neurology: 29-32 Late infantile metachromatic leukodystrophy (Mld) was successfully diagnosed in utero by demonstrating the absence of arylsulfatase-A in amniotic fluid using diethylaminoethyl-Sepharose column chromatography. Diagnosis by amniotic fluid using an ion-exchange column is [...]
Laskin, D. L.; Pilaro, A. M.; Ji, S., 1986: Potential role of activated macrophages in acetaminophen hepatotoxicity ii. mechanism of macrophage accumulation and activation. Toxicology and Applied Pharmacology 86(2): 216-226 Treatment of rats with acetaminophen (1.2 g/kg) results in the accumulation of activated macrophages in the centrilobular regions of the liver. To study the mechanism [...]