The term copurification—especially in the study of chemistry and biochemistry—refers to the process of physical separation, using either chromatography or one of the many purification techniques. This process involves two or more substances of interest, which will then be separated from other substances that are considered to be contaminants. The substances involved in the process of copurification typically attract each other in order to form a noncovalent complex (for example, a protein complex).
In some cases, however, the fractionating of mixtures—especially when these mixtures have a large number of components (such as in the case of a cell lysate), chances are that some of the components might copurify even though they do not form noncovalent complexes. In the context of these cases, the term copurification can be used to refer to the process in which two biochemical activities or some other properties are isolated together after a purification procedure. But it is important to note that it is not always certain if a specific sample has been purified to homogeneity, which means that it contains only one molecular species or a single molecular complex. These activities or properties are like within the process of copurification, but the process cannot guarantee to reside on the same molecule or even in the same molecular complex.
Copurification procedures, including co-immunoprecipitation, are typically utilized to analyze the various interactions between proteins. Copurification is also used in the mapping of the interactome (the whole set of molecular interactions in cells) of a wide variety of living organisms.
Other purification techniques used in the study of biology and biochemistry include chromatography, which employs adsorption and desorption on a packed bed of a specific solid matter in order to purify various components of one feedstream.
This category contains scientific information on copurification, a specific process of physical separation, using either chromatography or one of the many purification techniques.
Scrimgeour K.G., 1984: Folylpolyglutamate synthetase from beef liver assay stabilization and characterization. Canadian Journal Of Biochemistry & Cell Biology: 495-506 A reliable assay for folylpolyglutamate synthetase was devised and tested. Conditions were established for the complete separation of glutamate and the tritium-labeled products on columns of Deae-cellulose. The availability of this assay aided in partially [...]
Sluss P.M., 1988: Follicle stimulating hormone fsh immunoactivity in porcine follicular fluid is not pituitary fsh. Endocrinology: 487-491 Two inhibitors of Fsh binding to receptor have been isolated from porcine follicular fluid and shown to have in vitro biological activity. These inhibitors were distinct separable entities with opposite biological effects (agonist and antagonist) on cultured [...]
Gay J.L., 1980: Fluxes and accumulation of ethirimol in haustoria of erysiphe pisi and protoplasts of pisum sativum cultivar onward. Annals Of Applied Biology: 283-294 Isolated purified fractions containing haustorial complexes and mesophyll protoplasts were used to investigate in vitro the relative affinities of the host-parasite interface and host for the mildew specific fungicide, ethirimol. [...]
Katz M.L., 1988: Fluorophores of the human retinal pigment epithelium separation and spectral characterization. Experimental Eye Research: 71-86 Ten fluorescent fractions originating from the chloroform extracts of retinal pigment epithelial (Rpe) cells of human donor eyes (ages 52-98 yr) have been separated and characterized by Uv-vis absorbance and corrected fluorescence spectroscopy. The semipurified fluorophores fall [...]
Wada O., 1988: Fluorometric determination of prostaglandins and thromboxane in plasma by high performance liquid chromatography. Japanese Journal Of Hygiene: 1064-1070 The high-performance liquid chromatography (Hplc) method for detecting prostaglandins(PGs) and thromboxane(Tx) which was reported previously was found to have a serious problem in the detection of PGs and T In the present study, we [...]
Vogel, F.; Lumper, L., 1983: Fluorescence labeling of nadph cytochrome p 450 reductase ec 184.108.40.206 with the mono bromomethyl derivative of syn 9 10 di oxa bimane. Biochemical Journal 215(1): 159-166 The kinetics of thiol-group alkylation in Nadph-cytochrome P-450 reductase during its inactivation by monobromobimane was studied using the fluorimetric determination of S-bimane-L-cysteine by high-performance [...]
Heller F O., 1984: Flavonol content of guard cell and mesophyll cell protoplasts isolated from vicia faba cultivar weisskernige hangdown leaves. Physiologia Plantarum: 356-362 Guard cells of the lower epidermis of leaflets of faba L. cv. Wei.beta.kernige Hangdown contain several kaempferol 3,7-O-glycosides. This was demonstrated for the 1st time by the use of isolated, highly [...]
“Diez C., 1986: Flavonol content of two wines one red and one white of valdepenas vintage. Sciences Des Aliments: 361-372 Two wines, one red and one white, of “”Valdepenas”” vintage, showed a different flavonol composition, according to the analysis carried out by thin layer chromatography. After concentration and acidic precipitation of the wines and partial [...]
Berezin I.V., 1986: Firefly luciferase as a protein lipid complex. Doklady Akademii Nauk Sssr: 231-233 Data were presented on the thin-layer chromatography of the lipid composition of a crude firefly extract and electrophoresis of homogenous luciferase. Highly purified luciferase and the crude firefly extract contained phospholipids and neutral lipids whose qualitative composition in luciferase and [...]
Haar J.L., 1979: Fine structural analysis of the effect of trypan blue on the visceral endoderm of the mouse egg cylinder. Acta Anatomica: 256-268 The effects of a maternal injection of trypan blue on primitive streak mouse embryos were studied with Em. Commercial trypan blue was purified by descending paper chromatography, and pregnant females received [...]