The process of column chromatography is often employed in the study of chemistry. This method is used to purify a wide variety of individual chemical compounds from mixtures of the compounds. Column chromatography is also typically used for preparative applications on scales ranging from micrograms up to kilograms. One of the main advantages of column chromatography is the disposability of the stationary phase used in the process, as well as the relatively low cost of the process itself. This disposability property is responsible for preventing cross-contamination in the mixtures that pass through the process. Column chromatography is also great for preventing the degradation of the stationary phase due to recycling.
Column chromatography uses the classic preparative chromatography column—a glass tube with a diameter of five millimeters to fifty millimeters, with a height of five centimeters up to a meter. This glass tube features a tap and usually some kind of filter—typically a glass frit or a glass wool plug, which is used in the prevention of the loss of the stationary phase—right at the bottom. There are two methods used to prepare a column: the wet method and the dry method.
In the wet method, a slurry will be prepared from the effluent. This slurry contains the stationary phase powder, which is then carefully poured into the glass column. One must be careful to avoid air bubbles. A solution of the specific organic material should be pipetted on top of the stationary phase, which will then be topped with a layer of sand or with a cotton or glass wool.
For the dry method, the column will be filled with dry stationary phase powder, which will then be followed by adding the mobile phase.
This category contains scientific information on column chromatography, which is used to purify a wide variety of individual chemical compounds from mixtures of the compounds.
Asukabe, H.; Murata, H.; Harada, K.; Suzuki, M.; Oka, H.; Ikai, Y., 1994: Improvement of chemical analysis of antibiotics. 2 Simultaneous determination of three polyether antibiotics in feeds using high-performance liquid chromatography with fluorescence detection. Journal of Agricultural and Food Chemistry 42(1): 112-117 A simultaneous determination of three polyether antibiotics (PEs), salinomycin, monensin, and lasalocid, [...]
Bartok, T.; Szalai, G.; Lorincz, Z.; Borcsok, G.; Sagi, F., 1994: High-speed RP-HPLC/FL analysis of amino acids after automated two-step derivatization with o-phthaldialdehyde/3-mercaptopropionic acid and 9-fluorenylmethyl chloroformate. Journal Of Liquid Chromatography. 17(20): 4391-4403 This paper describes an automated high-speed Rp-Hplc/Fl method, including two-step precolumn derivatization with o-phthaldialdehyde/3-mercaptopropionic acid (Opa/3-Mpa) for primary amino acids and with [...]
Tarbin, J. A.; Shearer, G., 1993: High-performance liquid chromatographic method for the determination of the dinitrocarbanilide component of nicarbazin in eggs with on-line clean-up. Journal Of Chromatography Biomedical Applications. 613(2): 354-358 A high-performance liquid chromatographic method for the determination of the dinitrocarbanilide component of the anti-coccidial drug nicarbazin has been developed. The drug was extracted [...]
Tarbin, J. A.; Shearer, G., 1993: High-performance liquid chromatographic method for the determination of the anthelmintic nitroxynil in cattle muscle tissue with on-line anion-exchange clean-up. Journal Of Chromatography Biomedical Applications. 613(2): 347-353 A high-performance liquid chromatogaphic method for the determination of the anthelmintic nitroxynil has been developed. The drug was extracted from cattle muscle tissue [...]
Liu, Hj; Chang, By; Yan, Hw; Yu, Fh; Liu, Xx, 1995: Determination of amino acids in food and feed by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate and reversed-phase liquid chromatographic separation. Journal of AOAC International 78(3): 736-744 A study of a new amino acid analysis method using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a precolumn derivatization reagent for the analysis [...]
Zhong, He; Hastings, Felton L.; Hain, Fred P., 1994: Detection and quantification of trace levels of carbaryl and its metabolites from Dendroctonus frontalis Zimmermann. Journal Of Agricultural & Food Chemistry. 42(4): 949-953 A direct and sensitive high-performance liquid chromatographic (Hplc) method was developed for the detection and quantitative analysis of carbaryl and metabolites at the [...]
Kok, W. T., 1994: Derivatization reactions for the determination of aflatoxins by liquid chromatography with fluorescence detection. Journal Of Chromatography B Biomedical Applications. 659(1-2): 127-137 Various derivatization methods for the fluorometric detection of aflatoxins after separation by Hplc are reviewed. In normal-phase chromatography the sensitivity for aflatoxins B-1 and B-2, was improved by using special [...]
Holcomb, M; Thompson, Hc Jr; Hankins, Lj, 1993: Analysis of fumonisin B1 in rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection. Journal of agricultural and food chemistry 41(5): 764-767 A new, sensitive, and stable method has been developed for the analysis of fumonisin B1 in rodent feed. Fumonisin B1, [...]
Bagheri, H.; Brouwer, E. R.; Ghijsen, R. T.; Brinkman, U. A. T., 1992: Trace-level enrichment of phenylurea herbicides from surface and drinking water coupled on-line with column liquid chromatography-thermospray mass spectrometry. Analusis. 20(8): 475-482 Trace enrichment of phenylurea herbicide on a 10 mm times 2.0 mm id precolumn packed with Plrp-S polystyrene-divinylbenzene copolymer or on [...]
Bulterman, Albert J.; Vreuls, Jolan J.; Ghiljsen, Rudy T.; Brinkman, Udo A. T., 1993: Selective and sensitive detection of organic contaminants in water samples by on-line trace enrichment-gas chromatography-mass spectrometry. Hrc (journal Of High Resolution Chromatography). 16(7): 397-403 Liquid chromatographic (Lc) type trace enrichment is coupled on-line with capillary gas chromatography (Gc) with mass spectrometric [...]