The process of column chromatography is often employed in the study of chemistry. This method is used to purify a wide variety of individual chemical compounds from mixtures of the compounds. Column chromatography is also typically used for preparative applications on scales ranging from micrograms up to kilograms. One of the main advantages of column chromatography is the disposability of the stationary phase used in the process, as well as the relatively low cost of the process itself. This disposability property is responsible for preventing cross-contamination in the mixtures that pass through the process. Column chromatography is also great for preventing the degradation of the stationary phase due to recycling.
Column chromatography uses the classic preparative chromatography column—a glass tube with a diameter of five millimeters to fifty millimeters, with a height of five centimeters up to a meter. This glass tube features a tap and usually some kind of filter—typically a glass frit or a glass wool plug, which is used in the prevention of the loss of the stationary phase—right at the bottom. There are two methods used to prepare a column: the wet method and the dry method.
In the wet method, a slurry will be prepared from the effluent. This slurry contains the stationary phase powder, which is then carefully poured into the glass column. One must be careful to avoid air bubbles. A solution of the specific organic material should be pipetted on top of the stationary phase, which will then be topped with a layer of sand or with a cotton or glass wool.
For the dry method, the column will be filled with dry stationary phase powder, which will then be followed by adding the mobile phase.
This category contains scientific information on column chromatography, which is used to purify a wide variety of individual chemical compounds from mixtures of the compounds.
Poe D.P., 1984: P n n dimethylaminophenylisothiocyanate as an electrochemical label for high performance liquid chromatographic determination of amino acids. Journal Of Chromatography: 279-288 Three aromatic isothiocyanates were surveyed as possible precolumn derivatizing agents for the electrochemical detection of amines and amino acids. From the isothiocyanates studied, -,N-dimethylaminophenylisothiocyanate (Dmapi) was chosen for further development as [...]
Kissinger P.T., 1982: Nitro aromatic reagents for determination of amines and amino acids by liquid chromatography electrochemistry. Journal Of Liquid Chromatography: 881-896 Trinitrobenzene sulfonic acid, 2,4-dinitrofluorobenzene and 2-chloro-3,5-dinitropyridine were compared in terms of properties relevant to use as precolumn derivatization labels for amino compounds. This included consideration of chemical characteristics as well as purely detector [...]
Frei R.W., 1985: Miniaturization of solid phase reactors for on line post column derivatization in narrow bore liquid chromatography. Chromatographia: 453-460 The use of solid-phase reactors for post-column derivatization in narrow-bore Hplc (1.0mm i.d. analytical columns) is evaluated. Two systems are described, viz. for the determination of N-methylcarbamate pesticides and for that of urea and [...]
Brinkman U.A.T., 1987: Metal loaded sorbents for selective on line sample handling and trace enrichment in liquid chromatography. Journal Of Liquid Chromatography: 617-634 Three different commercially available stationary phases containing a thiol, an 8-hydroxyquinoline (oxine), and a 2-amino-1-cyclopentene-1-dithiocarboxylic acid (Acda) functional group, were loaded with mercury (Hg(Ii)), platinum (Pt(Iv)) and silver (Ag(I)) ions. The phases [...]
Wainer I.W., 1988: Measurement of underivatized propranolol enantiomers in serum using a cellulose tris 3 5 dimethylphenylcarbamate high performance liquid chromatographic hplc chiral stationary phase. Pharmaceutical Research (new York): 187-189 A commercially available high-performance liquid chromatographic (Hplc) chiral stationary phase (Hplc-Csp) has been used to measure serum levels of d- and l-propranolol. The Hplc-Csp is [...]
Kamiya H., 1987: Measurement of tryptophan in peptides by acid hydrolysis in the presence of phenol and its application to the amino acid sequence of a sea anemone toxin. Agricultural & Biological Chemistry: 1607-1616 The addition of phenol (about 1%) to 6 M HCl largely prevented destruction of tryptophan during hydrolysis of peptides at 110.degree. [...]
Braunsteiner H., 1987: Measurement of glycated protein by a rapid and specific method for absolute quantification of lysine bound glucose. Clinical Chemistry: 1656-1659 We modified the liquid-chromatographic assay of Schleicher and Wieland (J Clin Chem Clin Biochem 1981;19:81-7) for measuring lysine-bound glucose in serum proteins, increasing its performance and practicality. After precipitating serum proteins from [...]
Pinkerton T.C., 1986: Liquid chromatography of tin reduced technetium hydroxyethylidene diphosphonate complexes for on line spectral characterization and double isotope labeling. Analytical Chemistry: 1007-1013 An anion-exchange high-performance liquid chromatographic separation has been developed for tin-reduced technetium hydroxyethylidene diphosphonate complexes, Tc-Hedp, at millimolar concentrations of technetium-9 The separation facilitates the acquisition of ultraviolet-visible spectra of the [...]
Brinkman U.A.T., 1987: Liquid chromatographic trace enrichment with on line capillary gas chromatography for the determination of organic pollutants in aqueous samples. Journal Of High Resolution Chromatography & Chromatography Communications: 17-24 Trace enrichment for the Gc analysis of a series of chlorinated pesticides and polychlorinated biphenyls (PBCs) in aqueous samples has been achieved through a [...]
Frei R.W., 1984: Liquid chromatographic residue analysis of carbaryl based on a post column catalytic reactor principle and fluorigenic labeling. Analytical Letters: 915-932 A method was described for the residue analysis of carbaryl in polluted water samples. The technique was based on catalytic hydrolysis of the carbaryl on anion exchangers followed by fluorescence detection of [...]