The process of column chromatography is often employed in the study of chemistry. This method is used to purify a wide variety of individual chemical compounds from mixtures of the compounds. Column chromatography is also typically used for preparative applications on scales ranging from micrograms up to kilograms. One of the main advantages of column chromatography is the disposability of the stationary phase used in the process, as well as the relatively low cost of the process itself. This disposability property is responsible for preventing cross-contamination in the mixtures that pass through the process. Column chromatography is also great for preventing the degradation of the stationary phase due to recycling.
Column chromatography uses the classic preparative chromatography column—a glass tube with a diameter of five millimeters to fifty millimeters, with a height of five centimeters up to a meter. This glass tube features a tap and usually some kind of filter—typically a glass frit or a glass wool plug, which is used in the prevention of the loss of the stationary phase—right at the bottom. There are two methods used to prepare a column: the wet method and the dry method.
In the wet method, a slurry will be prepared from the effluent. This slurry contains the stationary phase powder, which is then carefully poured into the glass column. One must be careful to avoid air bubbles. A solution of the specific organic material should be pipetted on top of the stationary phase, which will then be topped with a layer of sand or with a cotton or glass wool.
For the dry method, the column will be filled with dry stationary phase powder, which will then be followed by adding the mobile phase.
This category contains scientific information on column chromatography, which is used to purify a wide variety of individual chemical compounds from mixtures of the compounds.
Post L.C., 1984: Fluvoxamine metabolic fate in animals. Drug Metabolism & Disposition: 82-92 The metabolic fate in animals of the antidepressant compound fluvoxamine was investigated. The 14c-labeled drug was administered orally to dogs, rats, hamsters and mice, and excretion in urine and feces was measured. Chromatographic patterns of the urines were developed by high performance [...]
Aposhian H.V., 1986: Fluorometric determination of 2 3 dimercaptopropane 1 sulfonic acid and other dithiols by precolumn derivatization with bromobimane and column liquid chromatography. Journal Of Chromatography Biomedical Applications: 297-310 The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (Dmps), has stimulated the need for a sensitive and selective method for [...]
Novotny M., 1988: Fluorescence reagents for high sensitivity chromatographic measurements of primary amines. Analytical Chemistry: 1765-1769 Model fluorogenic reagents have been synthesized and evaluated towards the goal of ultrahigh-sensitivity chromatographic analysis of primary amines with laser-based detection. Chemical reactivity and spectral criteria were utilized to evaluate 12 potential reagents forming highly fluorescent isoindoles. 3-Benzoly-2-quinolinecarboxaldehyde has [...]
Hagberg H., 1986: Extracellular overflow of neuroactive amino acids during severe insulin induced hypoglycemia in vivo dialysis of the rat hippocampus. Journal Of Neurochemistry: 178-184 Hypoglycemia-evoked changes in levels of extracellular excitatory and inhibitory amino acids were studied using the microdialysis technique. A newly designed dialysis probe was inserted stereotaxically into the rat hippocampus. Animals [...]
Brown P.R., 1985: Evaluation of conditions for the high performance liquid chromatography determination of plasma amino acids using precolumn derivatization. Journal Of High Resolution Chromatography & Chromatography Communications: 243-247 For the determination of free amino acids in plasma, the conditions for precolumn derivatization of the amino acids and the chromatographic separation were examined. The isoindole [...]
Duncalf D., 1986: Electrochemical determination of histamine derivatized with o phthalaldehyde and 2 mercaptoethanol. Journal Of Chromatography Biomedical Applications: 6 High-performance liquid chromatography coupled with electrochemical detection was used to determine histamine following precolumn derivatization with o-phthalaldehyde (Opa) and 2-mercaptoethanol. The isoindole derivative which is obtained as reaction product was electrochemically active at a moderate [...]
Arvidsson T., 1987: Effects of system peaks in a column switching liquid chromatographic system for bioanalysis. Journal Of Chromatography: 9-58 A reversed-phase liquid chromatographic column-switching system was used for the determination of drugs and metabolites in blood plasma with a direct injection procedure. A short precolumn was used for enrichment of the drugs and clean-up [...]
Arvidsson T., 1988: Effects of drug protein binding on trace enrichment of drugs in blood plasma on short precolumns. Journal Of Chromatography: 353-362 A reversed-phase liquid chromatographic precolumn venting plug technique was used for the determination of drugs by direct injection of large volume (500.mu.l) blood plasma samples. Enrichment of the drugs from large plasma [...]
Al Humaidan A., 1987: Direct simultaneous measurement of chloramphenicol and its monosuccinate ester in microsamples of plasma by radial compression liquid chromatography. Clinical Chemistry: 1814-1816 A simple method of simultaneous analysis for chloramphenicol and chloramphenicol succinate in 10-.mu.L samples of plasma is described. We injected the plasma samples directly into a radial-compression liquid chromatograph equipped [...]
Lindroth P., 1982: Diel and depth variations in dissolved free amino acids and ammonium in the baltic sea determined by shipboard high pressure liquid chromatography analysis. Limnology & Oceanography: 336-347 A precolumn fluorimetric labeling method was tested for shipboard Hplc analysis of dissolved free amino acids (Dfaa) in natural waters. No sample preparation is required [...]